 |
|
Original Article
| ||||||
| Molecular characterization of vancomycin resistant Enterococcus faecium clinical isolate in Makkah, Saudi Arabia | ||||||
| Husam Aldeen Hassan Tahir1, Abdalla Ahmed2, KawtharAbdelgaleil Mohammed Salih3, Bashir Sirag4 | ||||||
|
1Student, College of Graduate Studies, Sudan University of Science and Technology, Khanoum State, Sudan 2Associate Professor, Microbiology Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia 3Assistant Professor, Immunology, Sudan University of Science and Technology, Khanoum State, Sudan 4Lecturer, Microbiology Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia | ||||||
| ||||||
|
[HTML Full Text]
[PDF Full Text]
[Print This Article] [Similar articles in PubMed] [Similar articles in Google Scholar] |
| How to cite this article |
| Tahir HAH, Ahmed A, Salih KAM, Sirag B. Molecular characterization of vancomycin resistant Enterococcus faecium clinical isolate in Makkah, Saudi Arabia. Edorium J Microbiol 2018;4:100009M08HT2018. |
|
ABSTRACT
|
|
Aims: The aims of this study is to characterize the mechanism of vancomycin resistance in clinical Enterococcus faecium isolate recovered from ICU patient in Al-Nour Specialized Hospital in Makkah. Methods: In this study, resistant vancomycin resistant isolate was studied using whole genome sequencing. The genome was sequenced using Illumina MiSeq and sequence data was used to predict both the antibiotics resistant genes and the sequence type. In addition, the sequence data was used to screen for plasmids and virulence genes. Results: This resistant isolate were found to be belonging to sequence type 80 and harboring all known VanA genes, which include VanA, VanX, VanH, VanR, VanS, VanY and VanZ genes. Conclusion: Whole genome sequence of Vancomycin resistant E. faecium clinical strain revealed that it belonged to ST-80 which is a worldwide distributed sequence type. The vancomycin resistance was found to be due to the presence of VanA gene cluster. Three plasmids were predicted, two of them reported from E. faecium strains in many countries. Relation between transposon Tn-1546 and E. faecium plasmids also have been reported. Keywords: Enterococcus faecium, Vancomycin resistant, Whole genome sequencing |
|
[HTML Full Text]
[PDF Full Text]
|
|
Author Contributions
Husam Aldeen Hassan Tahir – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published Abdalla Ahmed – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published Kawthar Abdelgaleil Mohammed Salih – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published Bashir Sirag – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published |
|
Guarantor of Submission
The corresponding author is the guarantor of submission. |
|
Source of Support
None |
|
Consent Statement
Written informed consent was obtained from the patient for publication of this study. |
|
Conflict of Interest
Author declares no conflict of interest. |
|
Copyright
© 2018 Husam Aldeen Hassan Tahir et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information. |
|
|